Two Mutations Convert Mammalian Xanthine Oxidoreductase to Highly Superoxide-productive Xanthine Oxidase
نویسندگان
چکیده
منابع مشابه
Two mutations convert mammalian xanthine oxidoreductase to highly superoxide-productive xanthine oxidase.
Reactive oxygen species are generated by various systems, including NADPH oxidases, xanthine oxidoreductase (XOR) and mitochondrial respiratory enzymes, and contribute to many physiological and pathological phenomena. Mammalian xanthine dehydrogenase (XDH) can be converted to xanthine oxidase (XO), which produces both superoxide anion and hydrogen peroxide in a molar ratio of about 1:3, dependi...
متن کاملSpectrophotometric Assay for Superoxide Dismutase Based on the Reduction of Highly Water-soluble Tetrazolium Salts by Xanthine-Xanthine Oxidase.
Two novel highly water-soluble tetrazolium salts, WST-1 (4-[3-(4-iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate sodium salt) and WST-8 (4-[3-(2-methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate sodium salt) were applied to the assay of superoxide dismutase (SOD). The superoxide anion generated by xanthine/xanthine oxidase (XO) reduced WST-...
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The recently solved X-ray crystal structure of the molybdenum-containing arsenite ox idoreductase from Alcaligenes fa ecalis, is discussed, keeping in view the known mechanistic and spectroscopic information regarding the protein in a structural context. In addi tion, recent mechan istic studies of );,janthine oxidase are covered , with the conclusion that the reaction meclianism is initiated b...
متن کاملXanthine oxidase catalyzes the oxidation of hypoxanthine to xanthine and xanthine to uric acid as follows
The enzyme is well-suited to carry out the catalysis of these oxidative reactions, containing as it does four redox-active sites: molybdenum, flavin adenine dinucleotide (FAD) and a pair of iron-sulfur centers of the ferredoxin type (Fe2S2). The purine substrates interact with the enzyme at the molybdenum site of xanthine oxidase and oxygen at the flavin site. The two metabolic reactions above ...
متن کاملXanthine oxidase: introductory remarks.
At the 661st Meeting of the Biochemical Society, held at Bath, the Host Colloquium, a joint meeting with the Society for Free Radical Research, took place on 10th and 11th April 1997. Its title was ‘Xanthine oxidase: enzymology and pathophysiology’. The two aspects of xanthine oxidase research specified in the Colloquium title have developed remarkably separately, and, for a number of reasons, ...
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ژورنال
عنوان ژورنال: Journal of Biochemistry
سال: 2008
ISSN: 0021-924X
DOI: 10.1093/jb/mvn111